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Biomedical Imaging Group
3D Deconvolution Microscopy
BIG >  3D Deconvolution Microscopy
DECONVOLUTION

Outline

Open Software

» DeconvolutionLab2

» DeconvolutionLab

» PSF Generator

3D Reference Datasets

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Sinuosidal Siemens Star

2D Siemens Star.

Download

Object File Description Size Mb
Ground-truth ref.tif Reference dataset, simulation 256x256, 32 bits 67Mb
Simulation SIM.tif Test volume, reference convolves with the PSF, plus noise 256x256, 32 bits 33Mb
PSF psf.tif Point-Spread Function 256x128x128, 32 bits 33Mb

Reference

[1] D. Sage, L. Donati, F. Soulez, D. Fortun, G. Schmit, A. Seitz, R. Guiet, C. Vonesch, M. Unser, "DeconvolutionLab2 : An Open-Source Software for Deconvolution Microscopy" Methods, in press, 2017.

Results of LW+ - Landweber with positivity constraint


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Results of TRIF - Tikhonov Regularization Inverse Filter


Regularization 01

Regularization 02

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Regularization 15

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Regularization 17

Regularization 18

Regularization 19

Regularization 20

Conditions of use

You'll be free to use this software for research purposes, but you must not transmit and distribute it without our consent. In addition, you undertake to include a citation whenever you present or publish results that are based on it. EPFL makes no warranties of any kind on this software and shall in no event be liable for damages of any kind in connection with the use and exploitation of this technology.