| Tracking of Fluorescent Particles |
Investigators: Daniel Sage |
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Tracking single molecules has many applications in the fields of biophysics and cell biology. Among the most promising is the observation of intracellular molecular dynamics. |
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We have developed a robust algorithm for tracking a single fluorescent particle in dynamic image sequences obtained by brightfield or confocal microscopy. Specifically, we have considered the problem of extracting the movement of a fluorescently labeled chromosomal telomere within the nucleus of a budding yeast cell. Our method has three components
- an alignment module that compensates for the movement of the biological structure under investigation;
- an enhancement of particle-like structures by Mexican-hat filtering;
- a tracking module that uses dynamic programming to extract the optimal trajectory of the particle.
The experimental results obtained are quite promising. |
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Collaborations: Prof. Michael Unser, Prof. Susan Gasser (Molecular Biology Dept., UniGE) |
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Past Investigators: François Aguet |
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Funding: Grant application pending |
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[1] | D. Sage, F.R. Neumann, F. Hediger, S.M. Gasser, M. Unser, "Automatic Tracking of Individual Fluorescence Particles: Application to the Study of Chromosome Dynamics," IEEE Transactions on Image Processing, vol. 14, no. 9, pp. 1372-1383, September 2005.
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[2] | D. Sage, F. Hediger, S.M. Gasser, M. Unser, "Automatic Tracking of Particles in Dynamic Fluorescence Microscopy," Proceedings of the Third International Symposium on Image and Signal Processing and Analysis (ISPA'03), Rome, Italy, September 18-20, 2003, pp. I.582-I.586.
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