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Laboratoire d'imagerie biomédicale (LIB)
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On the Fundamental Limits of Nano-Particle Tracking Along the Optical Axis

F. Aguet, D. Van De Ville, M. Unser

Proceedings of the 2004 Annual Meeting of the Swiss Society of Biomedical Engineering (SSBE'04), Zürich ZH, Swiss Confederation, September 2-3, 2004, poster no. 30.


Tracking fluorescent nanoparticles is of particular interest for many applications in biology. Most tracking procedures try to reconstruct the particle's trajectory in an XY plane, or eventually in an XYZ volume when multiple acquisitions at different focal distances are available. In this work, we present the fundamental limits of recovering a nano-particle's position along the optical axis, from single or multiple plane z-stack acquisitions. In microscopy imaging, nano-particles can be considered as point sources with respect to the microscope's resolution. Therefore, the observed image at a certain out-of-focus distance corresponds to the microscope's defocused point spread function (PSF). Given such an image, it is possible to determine the axial position of a defocused particle. In this work, we show the restrictions and limitations on the precision of this estimation procedure as a function of common imaging parameters and noise statistics. We also show how taking into account the influence of using multiple acquisitions at different focal distances can increase the precision.

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AUTHOR="Aguet, F. and Van De Ville, D. and Unser, M.",
TITLE="On the Fundamental Limits of Nano-Particle Tracking Along the
	Optical Axis",
BOOKTITLE="2004 Annual Meeting of the Swiss Society of Biomedical
	Engineering ({SSBE'04})",
YEAR="2004",
editor="",
volume="",
series="",
pages="",
address="Z{\"{u}}rich ZH, Swiss Confederation",
month="September 2-3,",
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note="Poster no.\ 30")
© 2004 SSBE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from SSBE. This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder.
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