Sub-Resolution Maximum-Likelihood Based Localization of Fluorescent Nanoparticles in Three Dimensions
F. Aguet, D. Van De Ville, M. Unser
Proceedings of the Fourth IEEE International Symposium on Biomedical Imaging: From Nano to Macro (ISBI'07), Arlington VA, USA, April 12-15, 2007, pp. 932-935.
Several recent studies have shown that fluorescent particles can be localized with an accuracy that is well beyond traditional resolution limits. Using a theoretical model of the image formation process that accounts for possible sources of noise, Cramér-Rao bounds have been used to define the theoretical limits. A crucial influence on these bounds is the mismatch of refractive indices that is usually present between immersion medium and specimen. This results in an axially shift-variant point spread function, meaning that the bounds change as a function of the particle's position in the z-direction. We investigate the theoretical bounds for this shift-variant model, and propose a maximum-likelihood estimator for the particle position in 3D (XYZ position). Using this estimator, sub-resolution localization at the nanometer scale is demonstrated on experimental data. The results provide optimal conditions for particle tracking and localization experiments.
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AUTHOR="Aguet, F. and Van De Ville, D. and Unser, M.",
TITLE="Sub-Resolution Maximum-Likelihood Based Localization of
Fluorescent Nanoparticles in Three Dimensions",
BOOKTITLE="Proceedings of the Fourth {IEEE} International Symposium on
Biomedical Imaging: {F}rom Nano to Macro ({ISBI'07})",
YEAR="2007",
editor="",
volume="",
series="",
pages="932--935",
address="Arlington VA, USA",
month="April 12-15,",
organization="",
publisher="",
note="")