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An Introduction to Fluorescence Microscopy: Basic Principles, Challenges, and Opportunities

F. Aguet, C. Vonesch, J.-L. Vonesch, M. Unser

Microscopic Image Analysis for Life Science Applications, J. Rittscher, R. Machiraju, S.T.C. Wong, Eds., Artech House, Boston, pp. 85-114, 2008.


The discovery of fluorescent labels suitable for in vivo use and the development of high-resolution microscopes have transformed the fluorescence microscope into a mainstream tool, bringing about a revolution for biomedical imaging and having a profound impact on the way research is being conducted in the life sciences. Among a myriad of new imaging possibilities, subcellular components and processes can now be visualized in vivo, both structurally and functionally. Observations can be made in two or three dimensions, at different wavelength (spectroscopy), and possibly with time-lapse imaging, to investigate dynamic processes.

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AUTHOR="Aguet, F. and Vonesch, C. and Vonesch, J.-L. and Unser, M.",
TITLE="An Introduction to Fluorescence Microscopy: {B}asic Principles,
	Challenges, and Opportunities",
BOOKTITLE="Microscopic Image Analysis for Life Science Applications",
PUBLISHER="Artech House",
YEAR="2008",
editor="Rittscher, J. and Machiraju, R. and Wong, S.T.C.",
volume="",
series="Bioinformatics \& Biomedical Imaging",
type="",
chapter="4",
pages="85--114",
address="Boston MA, USA",
edition="",
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© 2008 Artech House Inc.. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from Artech House Inc.. This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder.
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