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An Introduction to Fluorescence Microscopy: Basic Principles, Challenges, and Opportunities

F. Aguet, C. Vonesch, J.-L. Vonesch, M. Unser

Microscopic Image Analysis for Life Science Applications, J. Rittscher, R. Machiraju, S.T.C. Wong, Eds., Artech House, Boston, pp. 85-114, 2008.



The discovery of fluorescent labels suitable for in vivo use and the development of high-resolution microscopes have transformed the fluorescence microscope into a mainstream tool, bringing about a revolution for biomedical imaging and having a profound impact on the way research is being conducted in the life sciences. Among a myriad of new imaging possibilities, subcellular components and processes can now be visualized in vivo, both structurally and functionally. Observations can be made in two or three dimensions, at different wavelength (spectroscopy), and possibly with time-lapse imaging, to investigate dynamic processes.


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