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3D Reconstruction and Comparison of Shapes of DNA Minicircles Observed by Cryo-Electron Microscopy

A. Amzallag, C. Vaillant, M. Jacob, M. Unser, J. Bednar, J.D. Kahn, J. Dubochet, A. Stasiak, J.H. Maddocks

Nucleic Acids Research, vol. 34, no. 18, pp. e125, September 29, 2006.


We use cryo-electron microscopy to compare 3D shapes of 158 bp long DNA minicircles that differ only in the sequence within an 18 bp block containing either a TATA box or a catabolite activator protein binding site. We present a sorting algorithm that correlates the reconstructed shapes and groups them into distinct categories. We conclude that the presence of the TATA box sequence, which is believed to be easily bent, does not significantly affect the observed shapes.

AUTHOR="Amzallag, A. and Vaillant, C. and Jacob, M. and Unser, M. and
        Bednar, J. and Kahn, J.D. and Dubochet, J. and Stasiak, A. and
        Maddocks, J.H.",
TITLE="{3D} Reconstruction and Comparison of Shapes of {DNA} Minicircles
        Observed by Cryo-Electron Microscopy",
JOURNAL="Nucleic Acids Research",
month="September 29,",

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