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Spontaneous Na+ Concentration Transients in Individual Mitochondria of Intact Astrocytes

G. Azarias, D. Van De Ville, M. Unser, J.Y. Chatton

Proceedings of the Lemanic-Neurosciences Annual Meeting (Diablerets'06), Les Diablerets VD, Swiss Confederation, September 8-9, 2006, pp. 58.



Astrocytes surrounding glutamatergic synapses remove released glutamate by a Na+-dependent cotransporter, which causes robust increases in cytosolic Na+ concentration. We recently showed using a fluorescent probe specific for mitochondrial Na+ (CoroNa Red), that Na+ increases occur also in the mitochondrial population where it is dynamically regulated [1]. Using the same fluorescent probe, we performed a study of individual mitochondria at the resting state and observed spontaneous transient increases in mitochondrial sodium (TIMS). We have developed an algorithm based on the wavelet transform to analyze and quantify TIMS in several pharmacological conditions. This study shows that individual mitochondria can exhibit dynamic regulation of their sodium content.


References

  1. Y. Bernardinelli, G. Azarias, J.-Y. Chatton, "In Situ Fluorescence Imaging of Glutamate-Evoked Mitochondrial Na+ Responses in Astrocytes," Glia, vol. 54, no. 5, pp. 460-470, October 2006.

Slides of the presentation (PDF, 812Kb)


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