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Extended Depth-of-Focus for Color Images in Bright-Field Microscopy

J. Berent, B. Forster, D. Van De Ville, D. Sage, M. Unser

Autumn Meeting of the Swiss Society of Pharmacology and Toxicology: Imaging Technologies and Their Applications in Pharmacology and Toxicology (ITAPT'03), Basel BS, Swiss Confederation, October 27-28, 2003.



Bright-field microscopy suffers from a small depth-of-focus. Typically, the specimen does not entirely lie in the focal plane and parts of the object that lie outside the focal plane are blurred. The specimen can be ‘scanned’ by moving the object along the optical axis and different images will contain different areas that are sharp. The purpose of image fusion is to combine those images into one single image with an extended depth-of-focus. One promising method is based on the wavelet transform. Basically, this approach computes the discrete wavelet transform of each image slice of the object, and constitutes the wavelets coefficients of the composite image by a maximum-absolute-value selection rule. The final composite image is obtained after computing the inverse wavelet transform. In this poster, we show how the wavelet-based image fusion technique can be successfully applied to obtain a sharp composite color image. The selection of the proper type of wavelets can improve the results. We also introduce a way to apply this technique for color images without introducing false colors. Various examples relevant to biomedical imaging are shown.


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