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Digital Holography Applied to Microscopy: A New Imaging Modality in the Sub-Wavelength Range

C. Depeursinge, É. Cuche, T. Colomb, P. Massatch, A. Marian, F. Montfort, M. Liebling, T. Blu, M. Unser, P. Marquet, P.J. Magistretti

Hundertvierte Jahrestagung der Deutschen Gesellschaft für angewandte Optik (DGaO), Münster, Federal Republic of Germany, June 10-14, 2003.



Digital holographic microscopy appears as a new imaging technique with high resolution and real time observation capabilities: longitudinal resolutions of a few nanometers in air and a few tenths of nanometers in liquids are achievable, provided that optical signals diffracted by the object can be rendered sufficiently large. Living biological cells in culture, have been observed with around 40 nanometers in height and half of a micron in width. The originality of our approach is to provide both a slightly modified microscope design, yielding digital holograms of microscopic objects and an interactive computer environment to easily reconstruct wavefronts from digital holograms.


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