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Extended Depth-of-Field for Color Images in Light Microscopy: Image Fusion and 3D Visualization

B. Forster, D. Van De Ville, J. Berent, N. Quack, D. Sage, M. Unser

Max Anliker memorial poster award, Proceedings of the 2004 Annual Meeting of the Swiss Society of Biomedical Engineering (SSBE'04), Zürich ZH, Swiss Confederation, September 2-3, 2004, poster 38.


Bright-field microscopy suffers from a relatively small depth-of-field. Typically, the specimen's profile covers a range larger than the depth-of-field, and parts of the specimen that lie outside the object plane appear blurred. The specimen can be ‘scanned’ by moving the object along the optical axis, and different images will contain different areas that are sharp. The purpose of image fusion is to combine those images into one single image with an extended depth-of-field. One promising method is based on the wavelet transform. We show how the wavelet-based image fusion technique can be successfully applied to obtain a sharp composite color image. The selection of the proper type of wavelets can improve the results. We also introduce a way to apply this technique for color images without introducing false colors. Finally, we show how height information on the specimen, which we obtain as a side product from our algorithm, can be used for 3D visualization.

Max Anliker Memorial Poster Award

@INPROCEEDINGS(http://bigwww.epfl.ch/publications/forster0403.html,
AUTHOR="Forster, B. and Van De Ville, D. and Berent, J. and Quack, N.
	and Sage, D. and Unser, M.",
TITLE="Extended Depth-of-Field for Color Images in Light Microscopy:
	{I}mage Fusion and {3D} Visualization",
BOOKTITLE="2004 Annual Meeting of the Swiss Society of Biomedical
	Engineering ({SSBE'04})",
YEAR="2004",
editor="",
volume="",
series="",
pages="",
address="Z{\"{u}}rich ZH, Swiss Confederation",
month="September 2-3,",
organization="",
publisher="",
note="Max Anliker memorial poster award")
© 2004 SSBE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from SSBE. This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder.
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