Biomedical Imaging GroupSTI
English only   BIG > Publications > Particle Reconstruction

 Home Page
 News & Events
 Tutorials and Reviews
 Download Algorithms

 All BibTeX References

Isotropic Resolution in Fluorescence Imaging by Single Particle Reconstruction

D. Fortun, P. Guichard, N. Chu, M. Unser

Proceedings of the Thirteenth IEEE International Symposium on Biomedical Imaging: From Nano to Macro (ISBI'16), Prague, Czech Republic, April 13-16, 2016, pp. 249-252.

Low axial resolution is a major limitation of fluorescence imaging modalities. We propose a methodology to achieve high isotropic resolution by reconstructing fluorescence volumes from observations of multiple particle replicates with different orientations. The challenge is to conciliate high reconstruction accuracy, requiring a large amount of input 3D data, with computational tractability. We achieve this goal by designing an iterative joint deconvolution and multiview reconstruction algorithm with an efficient augmented-Lagrangian based optimization. The computational cost is limited to only two FFTs per iterations, regardless of the number of input particles. We also adopt the nuclear norm of the Hessian as regularizer to avoid the usual staircase artifacts of the more standard total-variation. Experimental validation on realistic simulated data demonstrate the efficiency and accuracy of our method.

AUTHOR="Fortun, D. and Guichard, P. and Chu, N. and Unser, M.",
TITLE="Isotropic Resolution in Fluorescence Imaging by Single Particle
BOOKTITLE="Proceedings of the Thirteenth {IEEE} International Symposium
        on Biomedical Imaging: {F}rom Nano to Macro ({ISBI'16})",
address="Prague, Czech Republic",
month="April 13-16,",

© 2016 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from IEEE.
This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder.