Bi-Plane Calibration in Super-Resolution Microscopy
H. Kirshner, T. Pengo, N. Olivier, D. Sage, S. Manley, M. Unser
Proceedings of the Twelfth International Conference on Methods and Applications of Fluorescence Spectroscopy, Imaging and Probes (MAF'11), Strasbourg, French Republic, September 11-14, 2011, pp. 153.
We consider the task of aligning the imaging detectors of a bi-plane microscope for super-resolution applications . Such a microscope consists of two separate focal planes and the optical misalignment is modelled as an affine transform. In particular, u1 = A u2 + b accounts for translation, rotation and scaling operations (see illustration for an example of misaligned data). Here, u1, u2 describe a single point in space in terms of the two coordinate systems, A is a 3×3 matrix, and b is a translation vector. There are 12 misalignment parameters that need to be found, i.e. the matrix A and the vector b.
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AUTHOR="Kirshner, H. and Pengo, T. and Olivier, N. and Sage, D. and
Manley, S. and Unser, M.",
TITLE="Bi-Plane Calibration in Super-Resolution Microscopy",
BOOKTITLE="Proceedings of the Twelfth International Conference on
Methods and Applications of Fluorescence Spectroscopy, Imaging and
address="Strasbourg, French Republic",
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