Local Amplitude and Phase Retrieval Method for Digital Holography Applied to Microscopy

M. Liebling, T. Blu, É. Cuche, P. Marquet, C.D. Depeursinge, M. Unser

Proceedings of the SPIE European Conference on Biomedical Optics: Novel Optical Instrumentation for Biomedical Applications (ECBO'03), Münich, Germany, June 22-25, 2003, vol. 5143, pp. 210-214.

We present a numerical two-step reconstruction procedure for digital off-axis Fresnel holograms. First, we retrieve the amplitude and phase of the object wave in the CCD plane. For each point we solve a weighted linear set of equations in the least-squares sense. The algorithm has O(N) complexity and gives great flexibility. Second, we numerically propagate the obtained wave to achieve proper focus. We apply the method to microscopy and demonstrate its suitability for the real time imaging of biological samples.

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AUTHOR="Liebling, M. and Blu, T. and Cuche, {\'{E}}. and Marquet, P.
        and Depeursinge, C.D. and Unser, M.",
TITLE="Local Amplitude and Phase Retrieval Method for Digital
        Holography Applied to Microscopy",
BOOKTITLE="Proceedings of the {SPIE} European Conference on
        Biomedical Optics: {N}ovel Optical Instrumentation for Biomedical
        Applications ({ECBO'03})",
YEAR="2003",
editor="Boccara, A.-M.",
volume="5143",
series="",
pages="210--214",
address="M{\"{u}}nich, Germany",
month="June 22-25,",
organization="",
publisher="",
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