Biomedical Imaging GroupSTI
English only   BIG > Publications > Particle Tracking

 Home Page
 News & Events
 Tutorials and Reviews
 Download Algorithms

 PDF not available
 PS not available
 All BibTeX References

Automatic Tracking of Particles in Dynamic Fluorescence Microscopy

D. Sage, M. Unser

Autumn Meeting of the Swiss Society of Pharmacology and Toxicology: Imaging Technologies and Their Applications in Pharmacology and Toxicology (ITAPT'03), Basel BS, Swiss Confederation, October 27-28, 2003.

We present a new, robust algorithm for tracking fluorescent particles in dynamic image sequences obtained by brightfield or confocal microscopy. Specifically, we consider the problem of extracting the movement of chromosomal telomeres within the nucleus of a budding yeast cell. Our method has three components. The first is an alignment module that compensates for the movement of the biological structure under investigation. In our application, the images are aligned to the center of gravity of the nucleus which is detected by thresholding and fitted with an ellipse. The second step is a Mexican-hat filtering which we show to be optimally tailored to the detection of a Gaussian-like spot in fractal noise. The final component is a tracking algorithm that uses dynamic programming to extract the optimal (x, y, t) trajectory of a particle.

We have implemented the method as a Java Plugin for the public-domain ImageJ software. We have applied it to real data and have obtained results that are as good—if not better—as manual tracings. Our new algorithm reduces the analysis time of a 300 image sequence from 10 minutes, when it is done manually, to just a few seconds and offers the benefit of reproducibility.

AUTHOR="Sage, D. and Unser, M.",
TITLE="Automatic Tracking of Particles in Dynamic Fluorescence
BOOKTITLE="Autumn Meeting of the Swiss Society of Pharmacology and
        Toxicology: {I}maging Technologies and Their Applications in
        Pharmacology and Toxicology ({ITAPT'03})",
address="Basel BS, Swiss Confederation",
month="October 27-28,",

© 2003 SSPT. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from SSPT.
This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder.