Biomedical Imaging GroupSTI
English only   BIG > Publications > Fluorescence Microscopy

 Home Page
 News & Events
 Tutorials and Reviews
 Download Algorithms

 All BibTeX References

Computational Methods for High Resolution 3D Fluorescence Microscopy

D. Sage

Invited talk, First BioImage Analysis Community Conference "Workflows and Open Tools" (NEUBIAS2020'17), Lisbon, Portuguese Republic, February 12-17, 2017.

Epifluorescence microscopy results in blurry images with a very coarse optical sectioning; this limits its usefulness for cellular imaging, where resolving subcellular structures requires resolution close to or even beyond Abbe's diffraction limit. Techniques such as confocal microscopy (CLSM) and selective plane illumination microscopy (SPIM) have been proposed to reduce the out-of-focus light and to improve the resolution. Several other modalities, such as single-molecule localization microscopy (SMLM) and structured illumination microscopy (SIM) make the use of multiple acquisitions, trading time for resolution. These last techniques have been recently extended to 3D imaging.

AUTHOR="Sage, D.",
TITLE="Computational Methods for High Resolution 3D Fluorescence
BOOKTITLE="First BioImage Analysis Community Conference ``Workflows and
        Open Tools'' ({NEUBIAS2020'17})",
address="Lisbon, Portuguese Republic",
month="February 12-17,",
note="Invited talk")

© 2017 NEUBIAS. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from NEUBIAS.
This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder.