A Multiresolution Approach to Orientation Assignment in 3D Electron Microscopy of Single Particles
C.O.S. Sorzano, S. Jonić, C. El-Bez, J.M. Carazo, S. De Carlo, P. Thévenaz, M. Unser
Journal of Structural Biology, vol. 146, no. 3, pp. 381-392, June 2004.
Three-dimensional (3D) electron microscopy (3DEM) aims at the determination of the spatial distribution of the Coulomb potential of macromolecular complexes. The 3D reconstruction of a macromolecule using single-particle techniques involves thousands of 2D projections. One of the key parameters required to perform such a 3D reconstruction is the orientation of each projection image as well as its in-plane orientation. This information is unknown experimentally and must be determined using image-processing techniques. We propose the use of wavelets to match the experimental projections with those obtained from a reference 3D model. The wavelet decomposition of the projection images provides a framework for a multiscale matching algorithm in which speed and robustness to noise are gained. Furthermore, this multiresolution approach is combined with a novel orientation selection strategy. Results obtained from computer simulations as well as experimental data encourage the use of this approach.
@ARTICLE(http://bigwww.epfl.ch/publications/sorzano0401.html,
AUTHOR="S{\'{a}}nchez Sorzano, C.{\'{O}}. and Joni{\'{c}}, S. and
El-Bez, C. and Carazo, J.M. and De Carlo, S. and Th{\'{e}}venaz, P.
and Unser, M.",
TITLE="A Multiresolution Approach to Orientation Assignment in {3D}
Electron Microscopy of Single Particles",
JOURNAL="Journal of Structural Biology",
YEAR="2004",
volume="146",
number="3",
pages="381--392",
month="June",
note="")