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A Multiresolution Approach to Orientation Assignment in 3D Electron Microscopy of Single Particles

C.Ó. Sánchez Sorzano, S. Jonić, C. El-Bez, J.M. Carazo, S. De Carlo, P. Thévenaz, M. Unser

Journal of Structural Biology, vol. 146, no. 3, pp. 381-392, June 2004.


Three-dimensional (3D) electron microscopy (3DEM) aims at the determination of the spatial distribution of the Coulomb potential of macromolecular complexes. The 3D reconstruction of a macromolecule using single-particle techniques involves thousands of 2D projections. One of the key parameters required to perform such a 3D reconstruction is the orientation of each projection image as well as its in-plane orientation. This information is unknown experimentally and must be determined using image-processing techniques. We propose the use of wavelets to match the experimental projections with those obtained from a reference 3D model. The wavelet decomposition of the projection images provides a framework for a multiscale matching algorithm in which speed and robustness to noise are gained. Furthermore, this multiresolution approach is combined with a novel orientation selection strategy. Results obtained from computer simulations as well as experimental data encourage the use of this approach.

AUTHOR="S{\'{a}}nchez Sorzano, C.{\'{O}}. and Joni{\'{c}}, S. and
        El-Bez, C. and Carazo, J.M. and De Carlo, S. and Th{\'{e}}venaz, P.
        and Unser, M.",
TITLE="A Multiresolution Approach to Orientation Assignment in {3D}
        Electron Microscopy of Single Particles",
JOURNAL="Journal of Structural Biology",

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