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A Multiresolution Approach to Orientation Assignment in 3D Electron Microscopy of Single Particles

C.O.S. Sorzano, S. Jonić, C. El-Bez, J.M. Carazo, S. De Carlo, P. Thévenaz, M. Unser

Journal of Structural Biology, vol. 146, no. 3, pp. 381-392, June 2004.


Three-dimensional (3D) electron microscopy (3DEM) aims at the determination of the spatial distribution of the Coulomb potential of macromolecular complexes. The 3D reconstruction of a macromolecule using single-particle techniques involves thousands of 2D projections. One of the key parameters required to perform such a 3D reconstruction is the orientation of each projection image as well as its in-plane orientation. This information is unknown experimentally and must be determined using image-processing techniques. We propose the use of wavelets to match the experimental projections with those obtained from a reference 3D model. The wavelet decomposition of the projection images provides a framework for a multiscale matching algorithm in which speed and robustness to noise are gained. Furthermore, this multiresolution approach is combined with a novel orientation selection strategy. Results obtained from computer simulations as well as experimental data encourage the use of this approach.

@ARTICLE(http://bigwww.epfl.ch/publications/sorzano0401.html,
AUTHOR="S{\'{a}}nchez Sorzano, C.{\'{O}}. and Joni{\'{c}}, S. and
	El-Bez, C. and Carazo, J.M. and De Carlo, S. and Th{\'{e}}venaz, P.
	and Unser, M.",
TITLE="A Multiresolution Approach to Orientation Assignment in {3D}
	Electron Microscopy of Single Particles",
JOURNAL="Journal of Structural Biology",
YEAR="2004",
volume="146",
number="3",
pages="381--392",
month="June",
note="")

© 2004 Elsevier. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from Elsevier. This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder.
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