BIOMEDICAL IMAGING GROUP (BIG)
Laboratoire d'imagerie biomédicale (LIB)

Elastic Image Registration with Applications to Proteomics

C.O.S. Sorzano, P. Thévenaz, I. Valdés, A. Beloso, M. Unser

Proceedings of the Fifth International Workshop on Information Optics (WIO'06), Toledo, Kingdom of Spain, June 5-7, 2006, pp. 300-309.

In proteomics, electrophoretic 2-D gels are used to separate the proteome according to the molecular weight and electrical charge of its constituents, which are proteins expressed by a cell or organ at a particular time and under specific conditions. One of the main applications is the analysis of differential expressions between different conditions for which certain (perhaps many) spots are present in one of the images, but not in the other. One of the difficulties of this analysis is that 2-D gels are affected by spatial distortions due to run-time differences and dye-front deformations, which results in images that are significantly dissimilar and that pose a challenging problem to image-registration algorithms. In this paper, we test the efficiency of a state-of-the-art elastic-registration algorithm that we had already introduced in the context of biomedical images [1]. We study here the registration of simulated 2-D gels with known expression patterns and deformations. We show that our algorithm is capable of handling such situations. The proposed algorithm is publicly available at http://bigwww.epfl.ch under the name UnWarpJ.

References

  1. C.Ó. Sánchez Sorzano, P. Thévenaz, M. Unser, "Elastic Registration of Biological Images Using Vector-Spline Regularization ," IEEE Transactions on Biomedical Engineering, vol. 52, no. 4, pp. 652-663, April 2005. 

@INPROCEEDINGS(http://bigwww.epfl.ch/publications/sorzano0601.html,
AUTHOR="S{\'{a}}nchez Sorzano, C.{\'{O}}. and Th{\'{e}}venaz, P. and
	Vald{\'{e}}s, I. and Beloso, A. and Unser, M.",
TITLE="Elastic Image Registration with Applications to Proteomics",
BOOKTITLE="Proceedings of the Fifth International Workshop on
	Information Optics ({WIO'06})",
YEAR="2006",
editor="",
volume="",
series="",
pages="300--309",
address="Toledo, Kingdom of Spain",
month="June 5-7,",
organization="",
publisher="",
note="")
© 2006 EURASIP. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from EURASIP. This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder.