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Elastic Image Registration of 2-D Gels for Differential and Repeatability Studies

C.O.S. Sorzano, I. Arganda-Carreras, P. Thévenaz, A. Beloso, G. Morales, I. Valdés, C. Pérez-García, C. Castillo, E. Garrido, M. Unser

Proteomics, vol. 8, no. 1, pp. 62-65, January 2008.


One of the main applications of electrophoretic 2-D gels is the analysis of differential responses between different conditions. For this reason, specific spots are present in one of the images, but not in the other. In some other occasions, the same experiment is repeated between 2 and 12 times in order to increase statistical significance. In both situations, one of the major difficulties of these analysis is that 2-D gels are affected by spatial distortions due to run-time differences and dye-front deformations, resulting in images that are significantly dissimilar not only because of their content, but also because of their geometry. In this technical brief, we show how to use free, state-of-the-art image registration and fusion algorithms developed by us for solving the problem of comparing differential expression profiles, or computing an “average” image from a series of virtually identical gels.

@ARTICLE(http://bigwww.epfl.ch/publications/sorzano0801.html,
AUTHOR="Sorzano, C.{\'{O}}.S. and Arganda-Carreras, I. and
	Th{\'{e}}venaz, P. and Beloso, A. and Morales, G. and Vald{\'{e}}s,
	I. and P{\'{e}}rez-Garc{\'{i}}a, C. and Castillo, C. and Garrido, E.
	and Unser, M.",
TITLE="Elastic Image Registration of \mbox{2-D} Gels for Differential
	and Repeatability Studies",
JOURNAL="Proteomics",
YEAR="2008",
volume="8",
number="1",
pages="62--65",
month="January",
note="")

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