3D SIM and measurements time-reallocation for scanning based systems: Introduction and preliminary results on these two problems
Emmanuel Soubies, EPFL STI LIB

Meeting • 14 March 2017

Abstract
In this presentation, I will present preliminary results on two problems. The first problem concerns super-resolution image reconstruction from 3D structured illumination microscopy acquisitions. After an introduction of this microscopy modality, I will present how we can address the inverse problem using our inverse problem library. This setup being well suited to switch from a regularizer to another one, I will then compare the efficiency of different regularizers using simulated data. The second problem deals with measurements time-reallocation of scanning based systems. Current time-lapse image processing methods assume that each frame of the recorded time-series have been acquired within the same snapshot. Such an assumption is not true for scanning based systems which can result in a loss of both spatial and temporal resolutions of the reconstructed 2/3D+t sequences and can lead to a misinterpretation of underlying biological processes. The idea is thus to develop numerical reconstruction methods that solve the reconstruction problem by correctly reallocating measurements within the space-time domain.