PSF-Extractor: from fluorescent beads measurements to continuous PSF model
Emmanuel Soubies, EPFL STI LIB

Meeting • 11 September 2018

Abstract
I will present some results related to an ongoing work aiming at estimating a continuous point spread function (PSF) model from fluorescent beads measurements. In contrast to traditional methods, the proposed approach takes into account the real size of the beads (i.e. no point source assumption) and jointly estimates the beads positions (grid free) and the continuous PSF model. This presentation will also be an opportunity to present the last developments I have done in the library to deal properly with this kind of joint optimization approaches.