EPFL | Biomedical Imaging Group

3D PSF Models for Fluorescence Microscopy in ImageJ

H. Kirshner, D. Sage, M. Unser

Proceedings of the Twelfth Conference on Methods and Applications of Fluorescence Spectroscopy, Imaging and Probes (MAF'12), Strasbourg, France, September 11-14, 2011, in press.

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We introduce an ImageJ application that allows one to generate various 3D PSF models. Keeping the biological practitioner in mind, few input parameters are required only (Figure 1). Our application can generate z-stacks at any size and at any lateral and axial resolution. Our implementation utilizes a multi-thread design that allows for parallel and fast computations.

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