BIOMEDICAL IMAGING GROUP (BIG)
Laboratoire d'imagerie biomédicale (LIB)

Spontaneous Na+ Concentration Transients in Individual Mitochondria of Intact Astrocytes

G. Azarias, D. Van De Ville, M. Unser, J. Chatton

Proceedings of the CHUV Research Day (CHUV'07), Lausanne VD, Swiss Confederation, February 1, 2007, pp. 194.

Astrocytes surrounding glutamatergic synapses remove released glutamate by a Na+-dependent cotransporter, which causes robust increases in cytosolic Na+ concentration (Na+cyt). We recently showed using a fluorescent probe specific for mitochondrial Na+ (CoroNa Red), that Na+ increases occur also in the mitochondrial population where it is dynamically regulated. In the present study, we report that mitochondria of astrocytes can exhibit individual, spontaneous, and fully reversible increases in their Na+ concentration. We developed an image analysis algorithm based on the wavelet transform that allowed us to quantify the frequency and intensity of fluctuations under different conditions. In a field of ∼30 astrocytes, up to 1000 fluctuations per minute were typically detected under control conditions. The fluctuation duration and amplitude for a single mitochondrion averaged 12.2±0.8sec and 35.5±3.2mM, respectively. Under the same experimental conditions, mitochondrial electrical potential did not present such fluctuations. However, mitochondrial Na+ fluctuations were abolished by mitochondrial depolarization and were modulated by the cellular energy status. The fluctuation frequency was diminished at low Na+cyt. Blockers of several pathways potentially mediating fluctuations (e.g. calcium uniporter, permeability transition pore) failed to inhibit them. However, fluctuations appear to be linked to cellular proton homeostasis, since cytosolic alkalinization increased fluctuation frequency, whereas inhibition of Na+/H+ exchanger decreased it. Finally, application of glutamate led to a small decrease in the frequency of fluctuations. This study shows that besides global Na+ responses at the population level, individual mitochondria exhibit highly dynamic and regulation of their Na+ content with a possible link to proton homeostasis. 

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AUTHOR="Azarias, G. and Van De Ville, D. and Unser, M. and Chatton, J.",
TITLE="Spontaneous ${\mathrm{Na}}^{+}$ Concentration Transients in
	Individual Mitochondria of Intact Astrocytes",
BOOKTITLE="CHUV Research Day ({CHUV'07})",
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pages="194",
address="Lausanne VD, Swiss Confederation",
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