EPFL | Biomedical Imaging Group | Intracellular Nanomanipulator

Haptic Three-Dimensional Intracellular Nanomanipulator

A. Kulik, P. Thévenaz, J. Lekki, M. Lekka

Proceedings of the Eighth European Nanomanipulation Workshop (ENW'23), Kraków, Republic of Poland, May 15-17, 2023, pp. 84.

Classical AFM based nanomanipulator may allow movement of objects on a surface, however is lacking 'pick and release' function.

We have built a fully interactive three-dimensional (3-D) nanomanipulator integrated with an optical trapping-based Photonic Force Microscope (PFM) for in-vivo and in-situ operation inside living cells. A PFM is combined with a 3-D real-time haptic (force feedback) nanomanipulator. The system is based on an optical trap with an ultra-fast 3-D position detection system. It allows to manipulate in 3-D a probe inserted inside a living cell and to record its position with nanometer spatial and microsecond temporal resolution. By mapping the thermally induced movements of the probe, it is possible to measure intracellular forces and the 3-D stiffness matrix in real time. This instrument allow interactive accessing, positioning, measuring, imaging, and reacting.

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AUTHOR="Kulik, A. and Th{\'{e}}venaz, P. and Lekki, J. and Lekka, M.",
TITLE="Haptic Three-Dimensional Intracellular Nanomanipulator",
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