BIOMEDICAL IMAGING GROUP (BIG)
Laboratoire d'imagerie biomédicale (LIB)

Local Amplitude and Phase Retrieval Method for Digital Holography Applied to Microscopy

M. Liebling, T. Blu, É. Cuche, P. Marquet, C.D. Depeursinge, M. Unser

Proceedings of the SPIE European Conference on Biomedical Optics: Novel Optical Instrumentation for Biomedical Applications (ECBO'03), Münich, Federal Republic of Germany, June 22-25, 2003, vol. 5143, pp. 210–214.

We present a numerical two-step reconstruction procedure for digital off-axis Fresnel holograms. First, we retrieve the amplitude and phase of the object wave in the CCD plane. For each point we solve a weighted linear set of equations in the least-squares sense. The algorithm has O(N) complexity and gives great flexibility. Second, we numerically propagate the obtained wave to achieve proper focus. We apply the method to microscopy and demonstrate its suitability for the real time imaging of biological samples. 

@INPROCEEDINGS(http://bigwww.epfl.ch/publications/liebling0303.html,
AUTHOR="Liebling, M. and Blu, T. and Cuche, {\'{E}}. and Marquet, P.
	and Depeursinge, C.D. and Unser, M.",
TITLE="Local Amplitude and Phase Retrieval Method for Digital
	Holography Applied to Microscopy",
BOOKTITLE="Proceedings of the {SPIE} European Conference on
	Biomedical Optics: {N}ovel Optical Instrumentation for Biomedical
	Applications ({ECBO'03})",
YEAR="2003",
editor="Boccara, A.-M.",
volume="5143",
series="",
pages="210--214",
address="M{\"{u}}nich, Federal Republic of Germany",
month="June 22-25,",
organization="",
publisher="",
note="")

© 2003 SPIE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from SPIE. This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder.