BIOMEDICAL IMAGING GROUP (BIG)
Laboratoire d'imagerie biomédicale (LIB)

Artifacts in Optical Projection Tomography Due to Refractive-Index Mismatch: Model and Correction

Y. Liu, J. Dong, C. Schmidt, A. Boquet-Pujadas, J. Extermann, M. Unser

Progress in Biomedical Photonics (PBP'22), Lausanne VD, Swiss Confederation, December 6, 2022, poster no. 6.

Optical projection tomography (OPT) is a powerful tool for three-dimensional (3D) imaging of mesoscopic samples. While it is able to achieve resolution of a few tens of microns over a sample volume of several cubic centimeters, the reconstructed images often suffer from artifacts caused by inaccurate calibration. In this work, we focus on the refractive-index mismatch between the sample and the surrounding medium. We derive a 3D cone-beam forward model of OPT that approximates the effect of refractive-index mismatch. We then implement a fast and efficient reconstruction method to correct for the induced seagull-shaped artifacts on experimental images of fluorescent beads. 

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AUTHOR="Liu, Y. and Dong, J. and Schmidt, C. and Boquet-Pujadas, A. and
	Extermann, J. and Unser, M.",
TITLE="Artifacts in Optical Projection Tomography Due to
	Refractive-Index Mismatch: {M}odel and Correction",
BOOKTITLE="Progress in Biomedical Photonics ({PBP'22})",
YEAR="2022",
editor="",
volume="",
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address="Lausanne VD, Swiss Confederation",
month="December 6,",
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note="poster no.\ 6")
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