BIOMEDICAL IMAGING GROUP (BIG)
Laboratoire d'imagerie biomédicale (LIB)

Artifacts in Optical Projection Tomography Due to Refractive-Index Mismatch: Model and Correction

Y. Liu

International Symposium on Computational Sensing (ISCS'23), Luxembourg, Grand Duchy of Luxembourg, June 12-14, 2023.

Optical projection tomography (OPT) is a powerful tool for 3D imaging of mesoscopic samples. While it is able to achieve resolution of a few tens of microns over a sample volume of several cubic centimeters, the reconstructed images often suffer from artifacts caused by inaccurate calibration. In this work, we focus on the refractive-index mismatch between the sample and the surrounding medium. We derive a 3D cone-beam forward model of OPT that approximates the effect of refractive-index mismatch. We then implement a fast and efficient reconstruction method to correct for the induced seagull-shaped artifacts on experimental images of fluorescent beads. 

@INPROCEEDINGS(http://bigwww.epfl.ch/publications/liu2302.html,
AUTHOR="Liu, Y.",
TITLE="Artifacts in Optical Projection Tomography Due to
	Refractive-Index Mismatch: {M}odel and Correction",
BOOKTITLE="International Symposium on Computational Sensing
	({ISCS'23})",
YEAR="2023",
editor="",
volume="",
series="",
pages="",
address="Luxembourg, Grand Duchy of Luxembourg",
month="June 12-14,",
organization="",
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