BIOMEDICAL IMAGING GROUP (BIG)
Laboratoire d'imagerie biomédicale (LIB)

Optical Diffraction Tomography from Single-Molecule Localization Microscopy

T.-A. Pham

Single Molecule Localization Microscopy Symposium (SMLMS'21), Lausanne VD, Swiss Confederation, August 31-September 2, 2021.

Single-molecule localization microscopy (SMLM) is an imaging modality that delivers nanoscale resolution. It does so by sequentially activating a subset of fluorescent tags and by extracting their super-resolved positions from the microscope images. The emission patterns of individual tags can be distorted by the refractive-index (RI) map of the sample, which reduces the accuracy of the molecule localization if not accounted for.

In this work, we show that one can exploit those sample-induced aberrations to reveal the structural information of the specimen. Our work is related to the optical diffraction tomography in that we aim to recover the RI map. To that end, we propose an optimization framework in which we reconstruct the RI map and optimize the positions of the molecules in a joint fashion. The benefits of our method are twofold. On one side, we effectively recover the RI map of the sample. On the other side, we further improve the molecule localization—the primary purpose of SMLM. 

@INPROCEEDINGS(http://bigwww.epfl.ch/publications/pham2103.html,
AUTHOR="Pham, T.-A.",
TITLE="Optical Diffraction Tomography from Single-Molecule Localization
	Microscopy",
BOOKTITLE="Single Molecule Localization Microscopy Symposium
	({SMLMS'21})",
YEAR="2021",
editor="",
volume="",
series="",
pages="",
address="Lausanne VD, Swiss Confederation",
month="August 31-September 2,",
organization="",
publisher="",
note="")
© 2021 EPFL. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from EPFL. This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder.