Biomedical Imaging Group
Logo EPFL
    • Splines Tutorials
    • Splines Art Gallery
    • Wavelets Tutorials
    • Image denoising
    • ERC project: FUN-SP
    • Sparse Processes - Book Preview
    • ERC project: GlobalBioIm
    • The colored revolution of bioimaging
    • Deconvolution
    • SMLM
    • One-World Seminars: Representer theorems
    • A Unifying Representer Theorem
Follow us on Twitter.
Join our Github.
Masquer le formulaire de recherche
Menu
BIOMEDICAL IMAGING GROUP (BIG)
Laboratoire d'imagerie biomédicale (LIB)
  1. School of Engineering STI
  2. Institute IEM
  3.  LIB
  4.  Chemostat Array
  • Laboratory
    • Laboratory
    • Laboratory
    • People
    • Jobs and Trainees
    • News
    • Events
    • Seminars
    • Resources (intranet)
    • Twitter
  • Research
    • Research
    • Researchs
    • Research Topics
    • Talks, Tutorials, and Reviews
  • Publications
    • Publications
    • Publications
    • Database of Publications
    • Talks, Tutorials, and Reviews
    • EPFL Infoscience
  • Code
    • Code
    • Code
    • Demos
    • Download Algorithms
    • Github
  • Teaching
    • Teaching
    • Teaching
    • Courses
    • Student projects
  • Splines
    • Teaching
    • Teaching
    • Splines Tutorials
    • Splines Art Gallery
    • Wavelets Tutorials
    • Image denoising
  • Sparsity
    • Teaching
    • Teaching
    • ERC project: FUN-SP
    • Sparse Processes - Book Preview
  • Imaging
    • Teaching
    • Teaching
    • ERC project: GlobalBioIm
    • The colored revolution of bioimaging
    • Deconvolution
    • SMLM
  • Machine Learning
    • Teaching
    • Teaching
    • One-World Seminars: Representer theorems
    • A Unifying Representer Theorem

A Chemostat Array Enables the Spatio-Temporal Analysis of the Yeast Proteome

N. Dénervaud, J. Becker, R. Delgado-Gonzalo, P. Damay, A.S. Rajkumar, M. Unser, D. Shore, F. Naef, S.J. Maerki

Proceedings of the National Academy of Sciences of the United States of America, vol. 110, no. 39, pp. 15842-15847, September 24, 2013.


Observing cellular responses to perturbations is central to generating and testing hypotheses in biology. We developed a massively parallel microchemostat array capable of growing and observing 1,152 yeast-GFP strains on the single-cell level with 20 min time resolution. We measured protein abundance and localization changes in 4,085 GFP-tagged strains in response to methyl methanesulfonate and analyzed 576 GFP strains in five additional conditions for a total of more than 10,000 unique experiments, providing a systematic view of the yeast proteome in flux. We observed that processing bodies formed rapidly and synchronously in response to UV irradiation, and in conjunction with 506 deletion-GFP strains, identified four gene disruptions leading to abnormal ribonucleotide-diphosphate reductase (Rnr4) localization. Our microchemostat platform enables the large-scale interrogation of proteomes in flux and permits the concurrent observation of protein abundance, localization, cell size, and growth parameters on the single-cell level for thousands of microbial cultures in one experiment.

@ARTICLE(http://bigwww.epfl.ch/publications/denervaud1301.html,
AUTHOR="D{\'{e}}nervaud, N. and Becker, J. and Delgado-Gonzalo, R. and
	Damay, P. and Rajkumar, A.S. and Unser, M. and Shore, D. and Naef,
	F. and Maerki, S.J.",
TITLE="A Chemostat Array Enables the Spatio-Temporal Analysis of the
	Yeast Proteome",
JOURNAL="Proceedings of the National Academy of Sciences of the United
	States of America",
YEAR="2013",
volume="110",
number="39",
pages="15842--15847",
month="September 24,",
note="")

© 2013 PNAS. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from PNAS. This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder.
  • Laboratory
  • Research
  • Publications
    • Database of Publications
    • Talks, Tutorials, and Reviews
    • EPFL Infoscience
  • Code
  • Teaching
Logo EPFL, Ecole polytechnique fédérale de Lausanne
Emergencies: +41 21 693 3000 Services and resources Contact Map Webmaster email

Follow EPFL on social media

Follow us on Facebook. Follow us on Twitter. Follow us on Instagram. Follow us on Youtube. Follow us on LinkedIn.
Accessibility Disclaimer Privacy policy

© 2023 EPFL, all rights reserved