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SpotCaliper: Fast Wavelet-Based Spot Detection with Accurate Size Estimation

Z. Püspöki, D. Sage, J.P. Ward, M. Unser

Bioinformatics, vol. 32, no. 8, pp. 1278-1280, April 15, 2016.



Motivation: SpotCaliper is a novel wavelet-based image-analysis software providing a fast automatic detection scheme for circular patterns (spots), combined with the precise estimation of their size. It is implemented as an ImageJ plugin with a friendly user interface. The user is allowed to edit the results by modifying the measurements (in a semi-automated way), extract data for further analysis. The fine tuning of the detections includes the possibility of adjusting or removing the original detections, as well as adding further spots.

Results: The main advantage of the software is its ability to capture the size of spots in a fast and accurate way.

Availability and implementation: http://bigwww.epfl.ch/algorithms/spotcaliper/

Contact: zsuzsanna.puspoki@epfl.ch

Supplementary information: Supplementary data are available at Bioinformatics online.


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