BIOMEDICAL IMAGING GROUP (BIG)
Laboratoire d'imagerie biomédicale (LIB)

Artifacts in Optical Projection Tomography Due to Refractive-Index Mismatch: Model and Correction

Y. Liu, J. Dong, C. Schmidt, A. Boquet-Pujadas, J. Extermann, M. Unser

Optics Letters, vol. 47, no. 11, pp. 2618–2621, June 2022.

Optical projection tomography (OPT) is a powerful tool for three-dimensional (3D) imaging of mesoscopic samples. While it is able to achieve resolution of a few tens of microns over a sample volume of several cubic centimeters, the reconstructed images often suffer from artifacts caused by inaccurate calibration. In this work, we focus on the refractive-index mismatch between the sample and the surrounding medium. We derive a 3D cone-beam forward model of OPT that approximates the effect of refractive-index mismatch. We then implement a fast and efficient reconstruction method to correct for the induced seagull-shaped artifacts on experimental images of fluorescent beads. 

@ARTICLE(http://bigwww.epfl.ch/publications/liu2201.html,
AUTHOR="Liu, Y. and Dong, J. and Schmidt, C. and Boquet-Pujadas, A. and
	Extermann, J. and Unser, M.",
TITLE="Artifacts in Optical Projection Tomography Due to
	Refractive-Index Mismatch: {M}odel and Correction",
JOURNAL="Optics Letters",
YEAR="2022",
volume="47",
number="11",
pages="2618--2621",
month="June",
note="")

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